Egr-1 Luciferase Mouse Model - monitor and quantify Egr-1 activity in the living organism
The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis and cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism.
Our researchers developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under control of the murine Egr-1 promotor providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promotor activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy our researchers could show that Egr-1 promotor activity and Egr-1 mRNA levels were increased in the regenerating liver. In a model of wound healing it has been demonstrated that Egr-1 promotor activity was upregulated at the site of injury.
Taken together, a transgenic mouse model has been developed that allows real time in vivo imaging of the Egr-1 promotor activity. The ability to monitor and quantify Egr-1 activity in the living organism may facilitate a better understanding of Egr-1 function in vivo. The transgenic mouse model Egr-1-luc is available for licensing.
Live in vivo imaging of Egr-1 promoter activity during neonatal development, liver regeneration and wound healing Philipp Dussmann, Judith I Pagel, Sabina Vogel, Terese Magnusson, Rene Zimmermann, Ernst Wagner, Wolfgang Schaper, Manfred Ogris and Elisabeth Deindl, BMC Developmental Biology, 11:28 (20 May 2011)