Stable Isotope Labeled Peptides
Stable isotope labeled peptides, or SIL peptides, maintain identical amino acid sequence with the natural ones released from the parent proteins by chemical or enzymatic cleavage, but some of the amino acid residuals are labeled by specific stable isotope, such as 2H, 13C, and/or 15N atom(s).
Stable isotope labeled peptides have been widely applied in the nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). The combination of SIL peptides with NMR spectroscopy allow for the incorporation of NMR active nuclei which can reduce the complexity of spectra, and then help researchers to obtain novel correlations between atoms for more structural information. Meanwhile, the utilization of SIL peptides in quantitative proteomics can facilitate the comparison of different proteomes, and protein-protein interaction studies as internal standards, even MRM/SRM-based absolute quantification of specific target proteins in biological samples. This AQUA strategy, is quite useful in protein therapeutics discovery, pharmacokinetics, and toxicity studies, etc.
With more than 10 years of experience, and as a world-leading peptide manufacturing organization, Creative Peptides can provide customized peptides labeled with stable isotopes including 2H, 15N & 13C, respectively, or a combination of 15N & 13C. Even when the peptides are attached with specific modification motifs.